Unfortunately, as seiko has pointed out, ayahuasca blends can vary mmassively in composition and as a result we can't safely recommend a dose. I suggest checking the ayahuasca thread, found in the PD index (linked below) for some awesome information and absolutely not dosing until you've had a word with the person who made the blend. I've closed this thread.
Brewing ayahuasca generally cannot be smoked.