Manufacture of tryptophan derivatives with higher fungi

[yaesutom]

copy & pasted from a shroomery post,
------------

Manufacture of tryptophan derivatives with higher fungi
Gartz, Jochen
Akademie der Wissenschaften der DDR
Patent DD278600 (1990)

http://v3.espacenet.com/textdoc?IDX=DD278600&CY=ep&LG=en&DB=EPODOC


By culturing Psilocybe, Panaeolus, Inocybe, Conocybe, and Pluteus on solid media containing 0.1-2% 3-(2-dialkylaminoethyl)-indole, 3-(2-dialkylaminoethyl)-4-hydroxyindoles can be prepared in high yield with little synthesis of other indole compounds. P. cubensis was cultured on a cow manure-straw-water mixture containing 3-(2-dipropylaminoethyl)-indole.HCl. From 18 g mycelium, 420 mg 3-(2-dipropylaminoethyl)-4-hydroxyindole was isolated. No other indoles were detectable in the mycelium.


3-(2-dipropylaminoethyl)indole.HCl is DPT.HCl and 3-(2-dipropylaminoethyl)-4-hydroxyindole is 4-OH-DPT. On the patent they add 0.5 grams of DPT.HCl on the substrate and isolate 420 milligrams of 4-OH-DPT (I assume it is the freebase) with column chromatography from 18 grams of dry mushrooms (not mycelium, it looks like whoever wrote the abstract made a mistake).

Link in german

god dammit why won't it link/display at least..

ok another try:
http://mycellium.thruhere.net/


Anyone get the whole thing/translate it somehow? Sounds cool, 0.5g DPT in, and 0.42g 4-HO-DPT out..

 

[Morninggloryseed]

I'm on this one.

 

[Jamshyd]

...420 mg 3-(2-dipropylaminoethyl)-4-hydroxyindole was isolated. No other indoles were detectable in the mycelium.

What? Does this mean that the mushrooms decided that DPT is much more conveniet for their enzymes than DMT and abandoned all 4-hydroxylation of their endogynous DMT in favour of the DPT they were fed?

Or perhaps I don't get the DMT -> 4-HO-DMT conversion right?

This just doesn't sit right. And in fact, the pressence of 4-HO-DMT along with any biosynthesized 4-HO-DPT would taint the latter's experience, making this whole thing unworthwhile to me (Its a waste of Divine Powder).

 

[yaesutom]

Heh, can't save the pdf cause its like, secure 'n shit, well wow look! i found one thats not secure, and now you can just select all the text..

http://www.zodm.com/images/cls83008.pdf

Now, does anyone know the best german --> english translator for free to tranzzlate that shizznit?

hehe

 

[mapleleaf]

whats the point of this?? are they legal or something?

 

[yaesutom]

Maybe if you put enough DPT or whatever it gets 'overloaded' and ..possibly even prefers the structure of DPT better or something..

I'd love to grind up some cheap mimosa root bark and saturate it with DMT and see just how much psilocin you could get out of it. Actually also instead of using HCl etc for the acid to make the DMT salt, if you used phosphoric acid instead maybe result in more psilocybin?