Captain.Heroin
Bluelight Crew
My new friend/recent connect I met who exchanged 800-1000 needles just goes every few months with them and swaps. I;ve gone three times, each time I get 20 more than I can with. They will give you 20 for free without dropping anything and I usually drop some and ask for the extra 20 so I keep building up my supply since I switch between using their 28 Gs and buying 31Gs at the pharmacy so I keeo getting more and more. I wish they had 31G, when I went today the guy said that they didn't have them for me yet implying they were going to get some.
C.H.- I got my 10 pack of 10ml luer lock syringes with the plastic cannula tips for $8 shipped on ebay. At the exchange I found out they have 3ml luer lock syringes with 22g 1.5" needles plus 1ml syringes with 1" 25g needles that are luer lock but it slides onto the 1ml syringe which itself does not have a luer, so I'm going to use those needles with my 10mls.
Now I have a question about how you store your filtered solution, how do you keep it sterile if it is constantly being opened and reused? I was originally going to get resealable vials/media bottles but that concerns me. If you get the sterile sealed ones what does the top look like and how does it work? I know you need the needles to fill them but how do you fill them and extract solution if they are air tight? Are they vaccumm sealed? How many times can you draw fluid out of them before breaking the sterile seal? I think I would want to go with the vial route and make my solution batch and put it into 5 or so sterile vials that I could use for 4-7 days each if that would work. I am hesitant on using one big bottle that could easily get bacteria and other contaminants in it every time it is open to the air.
Your 10mL syringes are probably the most convenient to filter with; they can process the most volume at once.
Since I am currently using a media bottle, I can't exactly keep it sterile. I have to open it at least once a day or slightly less, so I just have to quickly draw what I need out of it and screw the top on. The lid screws on like a giant plastic coke 2 litre cap, except it stays pretty tight on there once it's on.
I'm going to use a vial next time, so that it'll stay sterile as long as I sterilize the rubber top every time I need to put in a new needle through the opening.
If you are concerned about contaminants in the air, I suggest buying a HEPA air filtration system. When you prepare your solution, you'll want the air to be clean in the environment you prepare it in anyways, so having a way to clean the air around where you're going to be working is a great thing.