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Minimum temp. to denature cannabis proteins?

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psood0nym

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It seems I've spontaneously developed an allergy to cannabis when used orally. In the past I've just mixed it with milk in oatmeal, microwaved, and eaten. But now that makes my chest hurt and my sinuses swell up. I suspect I'm allergic to proteins in the plant that are destroyed by heat, as I can smoke it without issue. Does anyone have an idea of what temperature I could heat cannabis to in an oven that would pretty much guarantee denaturing all the plant proteins without vaporizing the cannibinoids? Thanks.
 
Wow, that's crazy. Around a year ago same thing happened to me. It only happens when I touch the raw plant though. Like, licking a joint to roll it makes my lips puff up and my throat get itchy. However, eating a brownie seems to be fine. Also, smoking out of bongs or something with little actual contact to the weed is fine. Even if I'm breaking up bud for a blunt or something, and I scratch my eye\face, my eyes will swell up and get blood red and become itchy as FUCK. And yeah, this is before I smoke, so I'm not just 'mad high'.
 
Just because you denature a protein doesn't mean that you'll stop any immunological response. More likely it's a response to a non volatile component (as to what it is though is anybody's guess).

As a rule proteins denature below the boiling point of water, so microvawing with oatmeal should denature them, but you still get a bad response...
 
^ What he said. Take egg Allergy, if you're allergic to egg whites, then 99.9% of the time, you're allergy to one of the proteins. And it doesn't matter if the eggs are cooked or raw.

Why don't you do a butter and water extraction, then mix the butter in with the oatmeal (if you have such a penchant for weed oatmeal). If that still upsets your sinuses, then you know its a fat soluble compound with a boiling point above 100 degrees.
 
some of the proteins might re-fold after the temperature drops.

denaturing is a good idea in theory because its the epitopes that your immune system recognizes, which is why proteins are generally much more immunogenic than lipids or carbohydrates. but it just isnt working, so f*ck it.

i think the butter extraction is a good idea. just make a lot at once and save it.
 
Thanks for the responses. I thought it was a reaction to a protein in the cannabis plant because my understanding is that most plant food allergies are in reaction to a protein. My hope was that I could at least reduce the reaction to tolerable levels by denaturing the proteins. However, the last time I used cannabis orally it was an extraction into half-and-half milk, and I still had the reaction. Whether that's because the proteins got into the half-and-half and refolded or a non-volatile compound is responsible I don't know. Just thought I'd ask. Oh well. I may do a low dose experiment with butter instead of half-and-half over Christmas break just to be sure, as oral cannabis has much more interesting effects than smoked for me and it's worth mildly swollen sinuses to potentially salvage. I'll post back if I don't have any allergenic symptoms.
 
Sounds like it might be irritation due to the tiny crystals of calcium salts (mostly oxalate & carbonate), either mechanically (see dumbcane/mother in law's tounge for effects of calcium oxalate crystals - ouch!), or because calcium oxalate is poisonous - not that the amount contained on cannabis leaves is anywhere near the toxic level unless you eat a bale a day! - it precipitates out after contact with stomach acids liberate the oxalic acid wich combines with plasma Ca++. Youi have to eat rhubarb leaves or something similar to ingest toxic levels. Tea has tiny amounts of oxalic acid in and over years can result in some impressive kidney stones.

My twopennethworth - make it into an oil with alcohol (ethyl or isopropyl) & use that as the calcium salts should remain behind at the bottom of the jar with the alcohol in; obviously evaporate off the alcohol first unless you want to get drunk as well - isopropanol makes for a nauseous sort of drunken state, breathing acetone on everyone & is probably not what the smart people would ingest (I made an isopropanol extract to make up a cannabinoid drink with milk. Only ingested possibly 2ml but was sick as a dog!)
 
I would like some more information regarding some of the symptoms in the post made by NO W4RNING, if anyone has any as I seem to have the same problem.
If I touch cannabis then touch other parts of my body they swell, turn red, itch etc. This only applies to certain parts of the body however.
For example the palms of my hands are fine, but if I were to touch the back of my hands, arms, or anywhere else I experience the break out.
Seeing as I also smoke/eat/vaporize cannabis I would like to know if there could be any harm associated with it.
I don't seem to get any negative effects smoking or eating, but considering what it does to my skin, I'm quite curious.
Sorry if this isn't quite ADD material but I was reading the thread and considering the responses, and it seemed like I might be able to get the most useful information by asking here.
Thanks in advance for any input anyone may have. If this is the wrong place to ask I apologize to anyone who reads this and feels there time was wasted.
 
Has the OP considered various solvent extractions, ranging from hashish made from an alcohol wash or pressurized butane? These have varying degrees of purification, but even the simplest methods will remove a large amount of the plant materials and many can be rather simple though having varying degrees of waste.

Another angle that I'd consider would be the source, as he mentions suddenly developing the allergy. Obviously outdoor grown cannabis has measurable amounts of foreign pollens and other material, but even indoor grown can have a lot of problem organisms. Mold and fungus are quite common for some growing operations and can be tested for - as I understand it these are the biggest sources of negative impact for those with suppressed immune systems. There are labs in california and colorado that are doing this commercially, and suppliers that have pledged to check all of their product.

Even if these aren't a problem in your samples, many indoor operations don't use HEPA filters on their ventilation even though they should. And even if grown in filtered rooms without males, some amount of cannabis pollen is often present due to stress based or genetic hermaphroditism.

Said another way, have you changed up sources? What are your sources like in general?
 
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