endogenous cannabinoid activity?

[suark77]

Hi all-
I am a 31 year old male, 180lbs in very good shape.

I've recently been experimenting with various levels of endocannabinoids that aren't supposed to have any noticeable activity due to the fast action of MGL and FAAH. My hope is that someone else out there might have some additional input, that I might formulate a more reasonable ingestion/consumption table. I have been extremely skeptical of reports of the inactivity of endogenous ligands after a rather intense experience with 50mg IV phenethylamine (PEA)...and yes, I got the headaches. It was worth it.

Onwards:

Anandamide
1- baseline with empty stomach - oral, 25mg anandamide = nothing
2- baseline with empty stomach- oral, 50mg anandamide = nothing
3- baseline with empty stomach - oral, 100mg anandamide = slight euphoria, hypothermia, 20 minute duration...all effects gone after 1 hour.
4- baseline with empty stomach - oral , 150mg anandamide = noticeable euphoria NOT LIKE POT, duration 20 minutes?? no hangover. all effects gone after 1 hour.
5- baseline with empty stomach - oral, 200mg anandamide = intense euphoria, like a main-line plunger. hints of ZERO-anxiety marijuana is the only way to put it. music feels like playing on every cell in my body. i feel GOOD. duration 25 minutes, slight nitrus-like bubblehead for 1.5hrs, baseline. I could use this to go to sleep instantly.

2-arachidonoyl glycerol
1- baseline with empty stomach - oral, 25mg = nothing
2- baseline with empty stomach- oral, 50mg = nothing
3- baseline with empty stomach - oral, 100mg = nothing
4- baseline with empty stomach - oral , 150mg = nothing
5- baseline with empty stomach - oral, 200mg = some spinal shimmers, slight hypothermia, some slight cramping in stomach/intestines
6- baesline with empty stomach - oral, 300mg = there is something here, a decided calmness, but no "high"...I am eurphoric but unsedated. no stomach trouble, moderate 'munchies', no hypothermia. duration 30-35 minutes. ZERO hangover.
7- baesline with empty stomach - oral, 500mg = fuck all. stunning sense of warm, calm, not a dark edge in sight. wondrous body warmth that isn't sweaty. like falling asleep after a good night in Amsterdam, but no feeling of tiredness, more like lethargy - i can do anything, i just don't want to stir the moment. definate munchies. my thoughts race quickly for awhile, then drop off, some similarities to the first 10 minutes of really good pot, but unique, just not the same. duration 30 minutes. slight nitrus-like hangover for 3 hours until bedtime. THE NEXT DAY MY MUSCLES ARE A LITTLE SORE??? ESPECIALLY MY SHOULDERS???

human trial info seems lacking, save the 'chocolate' and 'oyster' stuff. anyone?

 

[MurphyClox]

I am really sceptical, because (as you already said), metabolic degradation is extremely fast here! Other means of application (at least the ones I can think of) don't promise better results.

I'm absolutely surprised by your 200 mg anandamide experience! Breakthrough?! Or just placebo? Could you repeat the experience?

Peace! Murphy

 

[shamus]

MGL & FAAH inhibitors anyone?

 

[Ham-milton]

mgl?

 

[negrogesic]

I think MGL is just another term for MAG-lipase...

Doesn't 500mg of 2-AG get expensive? It sounds certainly more interesting than anandamide, but are there any toxicity issues with such large doses of 2-AG?

 

[MurphyClox]

Doesn't 500mg of 2-AG get expensive? It sounds certainly more interesting than anandamide, but are there any toxicity issues with such large doses of 2-AG?

MGL = Monoacyl glycerol lipase (I guess )

I think toxicity isn't any problem here. The compounds are degraded rapidly and the resulting products are not toxic (being ethanolamine, glycerol, arachidonic acid)...at given concentrations.

 

[suark77]

"studies suggest that 2-AG may act as a high efficacy agonist, whereas 9-THC, WIN 55,212-2, and CP 55,940 are partial agonists" (Mackie et al., 1993; Shen, 1996; Sugiura and Waku, 2000).

repeated anandamide at 200mg, will not try more. i'm not a fucking monkey. full +++.

this stuff can be made in an oven by the OUNCE for less than $50 - du-ham-in-ate? does it smell like cleaning stuff in here, uncle fester?

anandamide is measured in pig brains (and even less in your brains) in the microgram. MICROGRAM. There's not enough FAAH/MGL (pick your poison from above) in a Viginia Ham Plantation to block/hydrolyze 200mg.

IMAGINE A WORLD WHERE A PILL GIVES YOU 100% OF THE EUPHORIA OF WEED, ONLY LASTS AS LONG AS LUNCH, AND IS PRACTICALLY IMPOSSIBLE TO A) OVERDOSE ON OR B) BUILD A TOLERANCE.

1-Evidence that the cannabinoid CB1 receptor is a 2-arachidonoylglycerol receptor. Structure-activity relationship of 2-arachidonoylglycerol, ether-linked analogues, and related compounds: T. Sugiura, et al.; J. Biol. Chem. 274, 2794 (1999) Full Text; http://www.jbc.org/cgi/reprint/274/5/2794
2-Enzymes of porcine brain hydrolyzing 2-arachidonoylglycerol, an endogenous ligand of cannabinoid receptors: S.K. Goparaju, et al.; Biochem. Pharmacol. 57, 417 (1999) Abstract
3-Metabolism of anandamide and 2-arachidonoylglycerol: an historical overview and some recent developments: V. Di Marzo, et al.; Lipids 34, S319 (1999), (Review) Abstract

 

[theWorldWithin]

suark77, no futher test at all? This sounds very interesting, have you considered writing a trip report for your +++ experience with anandamide? This is many times more interesting to me than the plethora of RC's floating around, what a massive potential for a safe rec substance.

 

[suark77]

noted

just tried this with my fourth friend last night. was too bleeped out to stay awake and write a decent summary during the experience. there is no cross tolerance. there is no tolerance. IT CAN LEAVE YOU MIGHTY TIRED IN THE A.M. i've started switching back and forth for 1.5-2 hrs (pop appropriate mg amount every 20 minutes), then go to sleep. it's as sure a thing as alcohol. the only thing that's hard is to stop. agonization is intense but brief, quite repeated with exactly the same effects every time. we (friends and i) all agree this is better than all known grown-up drugs, and then some. and yes, like many of you, we've run the gamut.

i don't know what the government is going to do. it's like pot just got legalized, only better. now i know where the -anand- came from. i get so stoned i start nodding out, totally smiling - and i hate opiates. it's like a spell of wonder is cast and then lifted. fucking israelis knew it the whole time and didn't share.

Since this is advance drug discussion, you guys know that this stuff can be made in barrels, literally, with the most common ingredients - fine i'll say it because most of you won't get it - ?PEANUTS?. Same precursor, absolutely everywhere worldwide. THIS IS NOT SYNTHESIS DISCUSSION, JUST LAYING THE GROUNDWORK FOR UNDERSTANDING HOW NATURAL THIS IS FOR HUMAN SUPPLEMENTATION.

i would like to give a shout out to murphyclox. peace.

 

[vecktor]

I would give this reported activity some credence if I thought the assayed material is what you claim. But for some inexplicable reason I have some doubts....

http://www.bluelight.ru/vb/showthread.php?t=375377

 

[Ham-milton]

wow... no kidding.
this is laughable, in light:

this stuff can be made in an oven by the OUNCE for less than $50

i could probably make it over a campfire with nothing but the 'smoking bubbling brooks' of the amazon's neighbors. but why bother, i've got a hotplate and stove-top vision corningware.

So your plan is to mix up peanut oil, with it's 6% arachidonic acid content? LOL

but then again, this is all just moronic:

IMAGINE A WORLD WHERE A PILL GIVES YOU 100% OF THE EUPHORIA OF WEED, ONLY LASTS AS LONG AS LUNCH, AND IS PRACTICALLY IMPOSSIBLE TO A) OVERDOSE ON OR B) BUILD A TOLERANCE.

If you want some credibility, don't act like an idiot.

A friend of mine tried 200mg of 2-AG IV the night he got me the AAI I asked for. I was intoxicated, he was sober. And we know for sure what he took.

 

[suark77]

nice try

1- x factor bodybuilding supplement from 'molecular nutrition' is the best place for bulk arachidonic acid 10%. FYI PEANUT OIL IS ONLY 1% OR SO ARACHIDONIC ACID, THE REST OF THAT 6% ARE THE OTHER FATTY ACIDS, BUT MOSTLY LINOLEIC. DO YOUR HOMEWORK BEFORE MAKING YOURSELF LOOK FOOLISH.

2- my chemistry has come along quite a bit. any response to my personal email, vektor? will you let me post it? this is me calling you out.

3- 2-AG... IV? dissolved in what, exactly? did his arm melt? i don't think so.

1-Evidence that the cannabinoid CB1 receptor is a 2-arachidonoylglycerol receptor. Structure-activity relationship of 2-arachidonoylglycerol, ether-linked analogues, and related compounds: T. Sugiura, et al.; J. Biol. Chem. 274, 2794 (1999) Full Text; http://www.jbc.org/cgi/reprint/274/5/2794

any questions?

 

[Ham-milton]

FYI PEANUT OIL IS ONLY 1% OR SO ARACHIDONIC ACID, THE REST OF THAT 6% ARE THE OTHER FATTY ACIDS, BUT MOSTLY LINOLEIC. DO YOUR HOMEWORK BEFORE MAKING YOURSELF LOOK FOOLISH.

Oh, thanks for making my point for me, re:

fine i'll say it because most of you won't get it - ?PEANUTS?. Same precursor, absolutely everywhere worldwide.

3- 2-AG... IV? dissolved in what, exactly? did his arm melt? i don't think so.

Ethanol. Not sure what your point is... sure, burned like hell, but his arm was fine.
edit: I should add that he also IV'd NADA later that evening, and ended up with a pretty bad migraine. He get's them occasionally, so it's not 100%, but I think it's fair to say it was the NADA and not an interaction with the two.


You might want to turn down the arrogance a bit ('calling you out' nonsense) Do you really think anyone would accept the validity of your experiments after you posted your kitchen chem questions re: the making of this?

Verify what's actually in the stuff and then someone might believe you.

 

[vecktor]

1- x factor bodybuilding supplement from 'molecular nutrition' is the best place for bulk arachidonic acid 10%. FYI PEANUT OIL IS ONLY 1% OR SO ARACHIDONIC ACID, THE REST OF THAT 6% ARE THE OTHER FATTY ACIDS, BUT MOSTLY LINOLEIC. DO YOUR HOMEWORK BEFORE MAKING YOURSELF LOOK FOOLISH.

2- my chemistry has come along quite a bit. any response to my personal email, vektor? will you let me post it? this is me calling you out.

3- 2-AG... IV? dissolved in what, exactly? did his arm melt? i don't think so.

1-Evidence that the cannabinoid CB1 receptor is a 2-arachidonoylglycerol receptor. Structure-activity relationship of 2-arachidonoylglycerol, ether-linked analogues, and related compounds: T. Sugiura, et al.; J. Biol. Chem. 274, 2794 (1999) Full Text; http://www.jbc.org/cgi/reprint/274/5/2794

any questions?

post what you like from the PM you sent me. keep synthetic details vague and its fine, recipes or 'how to' posts are verboten.

you might want to make sure of your facts before calling anyone out.
CAPS LOCK SUCKS TOO

I thought you might be interested in this
2 AG is 2-arachidonylglycerol, the reaction of arachidonic acid and glycerol mostly produces 1 AG not 2 AG, additionally glycerol has three alcohol groups the only way of ensuring monoacylation of glycerol is to use protection on the the unwanted groups.

Anandamide is arachidonyl ethanolamide not arachidonyl amide so requires monoethanolamine not ammonia. you might have discovered that the plain arachidonamide is active, which is highly interesting in itself. though I do not think that the amide is formed from the acid without activation of the acid. so you have rediscovered the highly potent drug placebomine.

vecktor

 

[MurphyClox]

Placebomine, yeah! LOL... Any synth details about that one?

 

[negrogesic]

Hold on, so none of you guys brew 2-AG over a campfire?

 

[vecktor]

Hold on, so none of you guys brew 2-AG over a campfire?

only on the weekends, and only when i'm not making lsd from moldy bread and fosters beer

 

[Jabberwocky]

man you guys just won't hesitate to rip into a fella will you?

 

[el_mago]

Dear esteemed enthusiasts,

Some of the best discoveries in pharma have been accidental artifacts...

The effect he describes seems pretty strong to be mistaken for placebomine.

Could it be that his no tech kitchen kookin has created an accidental bombshell that is destined to bring the world to its knees? I'm down for that...

You'll have to excuse the skepticism Suak77, but the world is flat my friend...
More details please so we may verify your results.

If this is all a hoax then Vektors ripping is quite justified, too many times have seen the perverted ego stroking pharmacohoaxca game play out...

If he has actually stumbled on the grail and we are grilling him in error, I think we all would gladly eat humble pie in exchange for the knowledge.

El_Mago

 

[vecktor]

Dear esteemed enthusiasts,

Some of the best discoveries in pharma have been accidental artifacts...

The effect he describes seems pretty strong to be mistaken for placebomine.

Could it be that his no tech kitchen kookin has created an accidental bombshell that is destined to bring the world to its knees? I'm down for that...

You'll have to excuse the skepticism Suak77, but the world is flat my friend...
More details please so we may verify your results.

If this is all a hoax then Vektors ripping is quite justified, too many times have seen the perverted ego stroking pharmacohoaxca game play out...

If he has actually stumbled on the grail and we are grilling him in error, I think we all would gladly eat humble pie in exchange for the knowledge.

El_Mago

I totally agree. I take a skeptical position because it doesn't hang true, extraordinary claims require extraordinary evidence, what he has is very unlikely to be 2-AG and is absolutely certain not to be anandamide.

I have no doubt that at some dosage level anandamide and 2 AG are active but I rather suspect that general toxicity will be noticeable at the required dose level, methanandamide is active without FAAH.