LSA extract, defatting etc

[slo mo]

Hi everyone%)

Seems like I have a grasp on the Teks out there but just a few details I could learn about below.

Is it counter productive to stir/shake the seed meat with the defatting agent too much and is it better to do so in a very cold atmosphere?
Is there a significant loss of LSA if the defatting process goes on too long with Petroleum Ether ?
If seeds are coated, what is the preferred way to wash and is it really effective allowing for a nice extract?
For the extraction, would there be much of a difference between 190 proof grain/ or cane vs 200 Proof ethanol (anhydrous undenatured)?
How long have you let the seed meat extract into the alcohol for and is it preferred to be done in the cold? For this step I would consider more stirring the better, and just let settle before the pull.

 

[Transform]

I've not done this and am not that familiar with the teks but since you have no answers I had a read of this one and will have a go at answering for you:

1. No, stirring helps things dissolve evenly. Higher temperatures make whatever is going to happen happen faster, and generally improves solubility too.
2. No, LSA is not supposed to be soluble in the defatting solvent at all.
3. I believe soap and water is the most effective method for removing any chemical coatings.
4. No, the 4% of water won't cause you much issue as both solvents are fairly similar.
5. I've not done it but I would say a good 30mins with stirring, less if you heated it. No open flames.

 

[slo mo]

I've not done this and am not that familiar with the teks but since you have no answers I had a read of this one and will have a go at answering for you:

1. No, stirring helps things dissolve evenly. Higher temperatures make whatever is going to happen happen faster, and generally improves solubility too.
2. No, LSA is not supposed to be soluble in the defatting solvent at all.
3. I believe soap and water is the most effective method for removing any chemical coatings.
4. No, the 4% of water won't cause you much issue as both solvents are fairly similar.
5. I've not done it but I would say a good 30mins with stirring, less if you heated it. No open flames.

Thanks Transform%)
1. Won't heat degrade the LSA? I read that LSA degrades at 20.2C (not sure how true it is, I have no texts on this) , but there was no duration/chart for that. If this stirring/defatting stage is done in a cool place (but with lots of stirring) will it still be very effective?
5. So for the extraction/pull, will stirring for longer periods possibly make the tincture effects less desirable, more lethargic ? Magnetic stirrer is something I would like to use (would it be beneficial?) , but for how long is what I am trying to determine. Real tests are probably the only way to know, but might as well ask.

Is there a way to determine which non polar solvent to chose for defatting ? D-limonene looks interesting, natural and it shouldn't remove LSA. I was thinking of Petroleum Ether before I read on a post where d-limonene could be used.

 

[Transform]

Heat will degrade it but there isn't a magical temperature where it starts to happen. All reactions happen at all temperatures, the exact temperature just determines how fast it will go. I would not concerned about running this reaction in an non polar solvent at elevated temperatures, maybe up to 40*C. Stirring while cool for a long time presents the same risks just at different reaction rates, but I would say that cooler temperatures are preferable in this case.

I don't think the timing will affect the subjective effects of the extraction, unless you think there is more than one active compound and one is being lost or destroyed?

A stirrer bar is pretty much the gold standard for mixing reactions. Convenient and effective.

Solvent selection is not really an exact science but I expect d-limonene would work quite well.