trainman04
Bluelighter
- Joined
- Dec 18, 2017
- Messages
- 189
adenylate cyclase is an enzyme with key regulatory roles in essentially all cells.
DNA > mRNA > Protein(CRH cells)
I did a search on SelfDeCode and it turns out I have 6 mutations for regulating Protein expression...
https://www.selfdecode.com/gene/mc4r/
''The mutant version of the MC4R gene produces a protein receptor that doesn't activate as well - it is less sensitive to signals from the body that tell the body that it is already full.''
https://www.selfdecode.com/gene/mtnr1b/
''The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity.''
https://www.selfdecode.com/gene/chrm3/
''It also inhibits adenylate cyclase''
https://www.selfdecode.com/gene/gabbr2/
''Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase.''
https://www.selfdecode.com/gene/adrb1/
''Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. ''
https://www.selfdecode.com/gene/fshr/
''The activity of this receptor is mediated by G proteins which activate adenylate cyclase.''
So this means due to all these mutations stacking up the adenylate cyclase which is supposed to regulate CRH is not being regulated.
I also have a mutation in my CRHR1 but not CRH or CRHR2. But CRHR1 can only be activated Via CRH right? So what would be causing high CRH to go trough CRHR1? Is it possible that due to the mutations not regulating the CRH protein cells and it somehow overactives CRHR1 which slowly made me more susceptible/prone to stress and the anxiety effect built up ? Is it possible due to the mutation in CRHR1 demands more CRH to be made? No idea or do I have a gene duplication in CRH/CRHR1?.. (doubt it)
I don't think I have a CRH-Secreting tumor either well atleast not in the brain tissues cause I did a brain scan but not full body anyhow outside of brain tissue it wouldn't affect me anyway.
Here are my CRHR1 mutations but I have no idea what they mean:
https://www.selfdecode.com/gene/crhr1/
https://www.selfdecode.com/gene/crhr1-it1/ (gene duplication?) <-? Intronic RNAs constitute the major fraction of the non-coding RNA in mammalian cells)
https://www.selfdecode.com/gene/mgc57346-crhr1/ (gene duplication?) <-? ADP-ribosylation factor)
can someone explain what the last two CRHR1 are?
Hm.....So I doubt I have gene duplication either , and no tumors either, and I don't think my body is making excess CRH mRNA. It is just my body is not enzyme (adenylate cyclase) is not regulating CRH protein expression..
it is possible to have low mRNA but high protein expression becuase a single mRNA molecule can stick around and be translated many times (which mine is becuase the enzyme is not regulating it!!!!!!!!)
Consider an enzyme: An mRNA molecule is translated to make a protein, which has enzymatic activity on some substrate, and can be regulated.
Expression is a readout for the translation of the mRNA into stable protein.
constitutive protein activity - "Constitutive" activity means that the regulation of the enzyme's activity is gone, it's been mutated or something to allow it to always be active.
So the text in Bold I believe is the issue, Are there any other things Im forgetting besides adenylate cyclase?
Becuase my DNA is fine then > mRNA is fine then when it is about to become protein the adenylate cyclase enzymes has to be active and able to regulate how much is becoming CRH protein...Now once it is an CRH protein I think the adenylate cyclase still can regulate it but the multiple mutation stacks are too many and the CRH becomes excess and now the CRHR1 mutations play a role and gets overactivated.
the questions:
1. Im just wondering is this possible could this be happening?
2. Its not possible for CRHR1 receptors to be activated any other way other than with its ligand CRH right? so no mutations that feed protein or something trough an loophole directly bypassing CRH?
DNA > mRNA > Protein(CRH cells)
I did a search on SelfDeCode and it turns out I have 6 mutations for regulating Protein expression...
https://www.selfdecode.com/gene/mc4r/
''The mutant version of the MC4R gene produces a protein receptor that doesn't activate as well - it is less sensitive to signals from the body that tell the body that it is already full.''
https://www.selfdecode.com/gene/mtnr1b/
''The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity.''
https://www.selfdecode.com/gene/chrm3/
''It also inhibits adenylate cyclase''
https://www.selfdecode.com/gene/gabbr2/
''Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase.''
https://www.selfdecode.com/gene/adrb1/
''Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. ''
https://www.selfdecode.com/gene/fshr/
''The activity of this receptor is mediated by G proteins which activate adenylate cyclase.''
So this means due to all these mutations stacking up the adenylate cyclase which is supposed to regulate CRH is not being regulated.
I also have a mutation in my CRHR1 but not CRH or CRHR2. But CRHR1 can only be activated Via CRH right? So what would be causing high CRH to go trough CRHR1? Is it possible that due to the mutations not regulating the CRH protein cells and it somehow overactives CRHR1 which slowly made me more susceptible/prone to stress and the anxiety effect built up ? Is it possible due to the mutation in CRHR1 demands more CRH to be made? No idea or do I have a gene duplication in CRH/CRHR1?.. (doubt it)
I don't think I have a CRH-Secreting tumor either well atleast not in the brain tissues cause I did a brain scan but not full body anyhow outside of brain tissue it wouldn't affect me anyway.
Here are my CRHR1 mutations but I have no idea what they mean:
https://www.selfdecode.com/gene/crhr1/
https://www.selfdecode.com/gene/crhr1-it1/ (gene duplication?) <-? Intronic RNAs constitute the major fraction of the non-coding RNA in mammalian cells)
https://www.selfdecode.com/gene/mgc57346-crhr1/ (gene duplication?) <-? ADP-ribosylation factor)
can someone explain what the last two CRHR1 are?
Hm.....So I doubt I have gene duplication either , and no tumors either, and I don't think my body is making excess CRH mRNA. It is just my body is not enzyme (adenylate cyclase) is not regulating CRH protein expression..
it is possible to have low mRNA but high protein expression becuase a single mRNA molecule can stick around and be translated many times (which mine is becuase the enzyme is not regulating it!!!!!!!!)
Consider an enzyme: An mRNA molecule is translated to make a protein, which has enzymatic activity on some substrate, and can be regulated.
Expression is a readout for the translation of the mRNA into stable protein.
constitutive protein activity - "Constitutive" activity means that the regulation of the enzyme's activity is gone, it's been mutated or something to allow it to always be active.
So the text in Bold I believe is the issue, Are there any other things Im forgetting besides adenylate cyclase?
Becuase my DNA is fine then > mRNA is fine then when it is about to become protein the adenylate cyclase enzymes has to be active and able to regulate how much is becoming CRH protein...Now once it is an CRH protein I think the adenylate cyclase still can regulate it but the multiple mutation stacks are too many and the CRH becomes excess and now the CRHR1 mutations play a role and gets overactivated.
the questions:
1. Im just wondering is this possible could this be happening?
2. Its not possible for CRHR1 receptors to be activated any other way other than with its ligand CRH right? so no mutations that feed protein or something trough an loophole directly bypassing CRH?
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