N&PD Moderators: Skorpio | thegreenhand
Br J Dermatol. 2011 Jun 28. doi: 10.1111/j.1365-2133.2011.10490.x.
Serotonin induces melanogenesis via serotonin receptor 2A.
Lee HJ, Park MK, Kim SY, Park Choo HY, Lee AY, Lee CH.
College of pharmacy, Dongguk University, Seoul 100-715, Korea Graduate School of East-West Medical Science, Kyung Hee University Global Campus, #1 Seocheon-dong, Giheung-gu, Yongin, Gyeonggi-do 446-701, Republic of Korea College of Pharmacy & Division of Life & Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, Republic of Korea Department of dermatology, College of medicine, Dongguk University, Ilsan Hospital, Goyang, Korea.
Background: The serotonin levels were increased by light exposure but the role and mechanism of serotonin in the pigmentation of skin cells are unclear. Objectives: To clarify the effect of serotonin on melanogenesis and to determine the serotonin receptor subtype involved. Methods: B16F10, SK-MEL-2 and Melan-a cells were cultured DMEM with low FBS. Three cell lines were treated with various concentration of serotonin (5-HT), 5-HT receptor agonists, and antagonists. The involvement of the 5-HT receptor 2A (5-HTR2A) was examined by gene silencing and 5-HTR2A antagonists. Results: 5-HT and the 5-HTR2A agonist, DOI increased the melanogenesis in three cell lines. These increased events were suppressed by 5-HTR2A antagonists or gene silencing of the 5-HTR2A gene. Conclusions: The serotonin receptor 5-HTR2A is involved in melanogenesis. These findings highlight the role of serotonin and suggest new ways of controlling melanogenesis.
I have a three-part question.
1. I am taking 200 mg of amisupride every morning. At this dosage it "preferentially block inhibitory pre-synaptic autoreceptors". What does that mean?
2. What will the effect of that dosage of amisulprider be with regards to various stimulants?
3. Somehow I seem to get more euphoria when I take amisulpride. Is that "right"? Or is it just placebo?
Hi guys,
Was hoping to get chemistry info about the pill-test kits. My understanding is limited. So (in the marquis) sulphuric acid acts as a catalyst and formaldehyde reacts with the compound to produce new compounds. These new compounds have colours? Can someone go into some detail?
Can someone think of an improved test kit? Would it be possible (theoretically) produce a test kit (or series of kits) that was able to make finer distinctions between compounds?
Thank you for the quick and precise answer. The stimulants I take are not prescribed, and my psychiatrist did not say anything about stimulants. I don't think she knows that's how amisulpride works....1. The axon of the pre-synaptic neuron stores, produces(?) and releases neurotransmitters into the synaptic cleft where they bind to receptors on the post-synaptic neuron, transmitting a signal. Autoreceptors are located on the pre-synaptic neuron and bind the same neurotransmitter, but the effect is generally to reduce the amount of neurotransmitter produced/released by the neuron, so they provide negative-feedback to regulate neurotransmitter release. Blocking dopamine autoreceptors will increase dopamine release and, therefore, dopaminergic neurotransmission.
2. I guess it would potentiate the dopaminergic component of stimulants.
3. That's the effect I'd expect, though I tried 100mg amisulpride and maybe 5mg aripiprazole and noticed no effect. Were you told it should have that effect when you started taking it?
Hi guys,
Was hoping to get chemistry info about the pill-test kits. My understanding is limited. So (in the marquis) sulphuric acid acts as a catalyst and formaldehyde reacts with the compound to produce new compounds. These new compounds have colours? Can someone go into some detail?
Can someone think of an improved test kit? Would it be possible (theoretically) produce a test kit (or series of kits) that was able to make finer distinctions between compounds?