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Limitation of Nitazene Test Strips

ShulginsReincarnation

Ex-Bluelighter
Joined
Dec 8, 2024
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Can anyone enlighten me as to the exact cross reactivity of the newer test strips that work for nitazenes? I am finding out more and more, bit by bit about them and their limitations. So, I am just now reading that they cross react with caffeine and heroin? Interesting to note if true, but can anyone confirm this? Also, can anyone elaborate more on this issue more generally speaking and what is being done to fix these problems?
 
There are at least two different brands on the market, different factories have different antibodies and this means they can have different cross-reactivity. We saw this with the fentanyl strips, whereby a new manufacturer was able to develop a fentanyl strip that did not react with MDMA/methamphetamine.

I don't think anywhere except the manufacturers have published information about cross reactivity. I think it would be really annoying if they were cross reactive with caffeine or heroin but these strips are SO sensitive to nitazenes, able to detect just a few molecules. So you can just dilute to under 1mg/mL and you'll get a real result.
 
There are at least two different brands on the market, different factories have different antibodies and this means they can have different cross-reactivity. We saw this with the fentanyl strips, whereby a new manufacturer was able to develop a fentanyl strip that did not react with MDMA/methamphetamine.

I don't think anywhere except the manufacturers have published information about cross reactivity. I think it would be really annoying if they were cross reactive with caffeine or heroin but these strips are SO sensitive to nitazenes, able to detect just a few molecules. So you can just dilute to under 1mg/mL and you'll get a real result.
Yes, the brand I handed out to a treatment center a few weeks ago were accurate at 20ng/mL I believe. Not sure about the next batch I am getting today, but I wonder about the cross reactivity of each brand now as I didn't even think to look which was my mistake. I was just most concerned that the people at risk were not even being taught about nitazenes because the people heading the departments of hospitals here aren't even aware of them being put in street drugs just yet it seems. They aren't even fully aware of the RC benzos yet either; I usually have to give the hospitals a decade or two to catch up it seems. I digress though....

Thank you for your thoughts. You have a good point here about sensitivity and variability depending on manufacturer as well which I will keep in mind from now on.
 
Yes, the brand I handed out to a treatment center a few weeks ago were accurate at 20ng/mL I believe. Not sure about the next batch I am getting today, but I wonder about the cross reactivity of each brand now as I didn't even think to look which was my mistake.

2000ng/ml more like. 1ug/ml

or are we are supposed to believe so there are test strips 2 orders of magnitude more sensitive than the BTNX Rapid Response lateral flow immunoassays examined here?

FWIW BTNX is the same as the nitazene strip sold by EZ test and practially everywhere. if there is something 100x better then BTNX should be discarded.

There are technical reasons why these lateral flow tests are competition not sandwich format this unfortunately means they are rather flawed.
Competition assays are where a latex bead labelled 'nitazene like compound' competes for sites on an anti nitazene antibody .
Positives are absence of test line. showing one line only. Negatives are 2 lines.
This format makes the test insensitive because it is harder to see than sandwich and open to false positives (anything blocking the test line including some pH changes will false positive) worse the nitazenes that do not bind to the antibody will false negative.

diluting samples to reduce false positives with a competition assay is not very smart because the interfering substances are going to be present in much higher amounts than the nitazenes so it turns a potential false positive into a false negative, which would you prefer?

speculating these strips appear to contain a nitazene analog or dummy compound bound to a colored latex bead via a linker attached to the benzimidazole nitrogen (in place of the NN-diethylaminoethyl in nitazenes) so they will fail to spot some of the nitazenes already out there in RC land. The contained antibody was probably made by attaching a hapten to the same imidazole nitrogen and then injecting some animals with it and bleeding them then killing them for their antibodies. For certain animals were harmed in the making of this product and the product don't work too good.
 
I think it worth reminding people that the nitazenes are a class rather than a specific drug. So IF you get a positive, it might be something x3 morphine, it migh be something x6000 morphine in potency. Some nitazenes don't even contain a nitro moiety and there seems to be an endless supply of new analogues.

In fact, they don't actually need to contain a benzimidazole system. I have read some papers which showed that an indole ring will produce an active opioid.


Voila - note how it was the US Army that developed the stuff and that no information is extant. Well, if I can find it, I presume other chemists can do the same.

It's getting really dangerous out there. Take care people.
 
Good work !

Real dangerous , NL Blanket Ban Law proposal.
Despite my expectation now real, so after the washout period over.

Disturbing was that 1/2 the labs the Police rolled up were 1/2 RC,
which they had to leave behind. And 1/2 illegal drug production,
i assume MDMA, Amphetamine maybe GHB.

So they have the RC lab stuff and staff well trained, jobless chemist.
Now the way and 1/2 is probably alreday in criminal hands.

A poster warned Meth-Amphetamine, Fentanyl analogues and Nitazenes.
From NL will spread through the whole EU. And well my Gouvernement.
Just finished a velvet pavement to work on. Hope i am wrong, it is going wrong.

You know we , the Dutch also declared the war on Drugs, pretty long after the US concluded it doesn t work. We are tribunal EU Parlement as the Dutch state poisons its citizen s and that is not allowed.

Later, super thread, gonna check what happened.
Did they get a :butt2:-whippin or not ?
 
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