Artificial Emotion
Bluelighter
Firstly, if a picture is worth a thousand words, then a video must be worth a million. Forgive the 'skunk' terminology and the scatty 'journalist', but here's an interesting experiment where the woman is given a THC intravenously on its own. In the same settings and conditions, another injection is given by the person conducting the experiment except this time CBD is also included in the injection of THC. The difference is striking, with the CBD making the experience far more enjoyable and even comical. So, strains with high CBD levels can be a godsend for people prone to anxiety and paranoia when smoking, such as myself:
Video of the effects of IV THS vs. IV CBD+THC
Anyway, as I was saying, you need to choose the right strain to start off with. Mazar-I-Sharif supplied by The Real Seed Company for example is a perfect choice, since it's a pure indica landrace strain that has the potential to produce high CBD plants in an indoor environment rather than tall, lanky sativas poorly suited to indoor growing.
So aftering thinking about it, here's what I would do: I would grow out a decent number of seeds to get the full complement of low and high CBD phenotypes, since a low number (5 for example) could result in say 2-3 females which might all be low CBD and a waste of time. A good number would be at least 18-20, but the more, the better, with about 20-25 being ideal. The next step would be to veg them out until the pre-flowers show, at which point cuttings would be taken from each plant and sexed using the Genefinder method. The clones identified as male would allow me to take the next step of removing all the males from the main grow area, or as in my case, moved to a dedicated male grow area. I would continue to grow the clones out so that I have all the different phenotypes to work with after I've harvested the main plants. These clones would be turned into bonsai mother plants since they take up less space and can be used to produce as many cuttings as required. They look pretty cool too!
So once I have a room full of females I would use a 12/12 schedule instead of an 18/6 or 20/4 schedule and flower them. Each would then be harvested and cured and stored in airtight mason jars labelled according to the plant from which each bud was harvested. It may be possible to determine which sample is high CBD just from the subjective high, with high CBD strains being less anxiety-inducing and more mellow, I don't know. If not however, there are relatively inexpensive tests widely available, such as the Canalyse kit which is a cannabinoid fingerprint testing kit. This gives you a qualitative analysis of the cannabinoid profile and will indicate whether the bud sample is high, medium or low in CBD levels compared to d-9-THC. I believe other tests can use standardised samples to give you a quantitative analysis, including d-9-THC percentage. These kits, without going into too much detail, use chromatography to give you an indication of relative cannabinoid levels present in the small bud samples being analysed. The Cannalyse kit is relatively cheap, but more expensive compared to the other kits on the market, which are often less than a quarter the cost of Cannalyse.
Anyway, after indentifying the high CBD phenotypes, I would label their clones. Since I believe the high CBD allele is recessive, I would simply select out the clone who's mother produced bud with high CBD levels. Since there are three possible phenotypes, cc, cC and CC, producing a population consisting of only high CBD phenotypes would require a cross between a male and female each consisting of cc genotypes. The female is easy to identify by smoking the bud, in theory, but the male containing the cc genotype can be identified indirectly since all offspring it produces when crossed with the female will also be high CBD. We would not have the difficulty of trying to distinguish between the cC and CC genotypes, each of which have the same phenotype, because there's only one genotype and one corresponding phenotype we're looking for which makes it a lot more simple.
But anyway, all you need to really do is select a high CBD phenotype and simply clone the female for future grows. This doesn't necessarily require TLC cannabinoid fingerprint analysis, since simply smoking the bud samples would suffice in practice. Once you have that one 'keeper' phenotype you never have to select another mother plant again. The fact that high CBD phenotypes are known to be present in any given population of Mazar-I-Sharif means that finding a high CBD keeper mum is highly likely in a pack of 12-20 seeds (half or thereabouts of which would be female), unlike most of the other Dutch-type non-landrae hybrids on the market at the moment, which have been selectively bred to have high THC levels, but low CBD levels at the same time albeit unintentionally.
edit: It seems that I have misinterpreted the function of the THC/CBD alleles in this case. This post explains it:
FYI:
ganga = herbal cannabis and
charas = hashish.
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if you dont mind, which antidepressant(s) did you take?![:\](https://bluelight.org/xf/BL_Images/Orig_Emoji/wrygrin.gif "wry grin :\")
