2 grams is a good starting dose for mushrooms, yes. But to answer your question, there will be some degree of tolerance. Probably not a lot, but it will be a little weaker than if you waited.
Sounds like you're new to psychedelics. It's one thing to take psychedelics 5 days apart on a special occasion, like if you're on vacation or a friend is visiting or something, but you shouldn't get into the habit. Over time you'll start to lose the magic if you don't give more time between your trips.