Im Not Dead.

[Psychonautical]

Hey folks its been a long time since ive posted anything on here.
ive been busy mastering the world of mycology because those goddamn 4-po organically derived versions of tryptamines have authentically and forever encapsulated my wanton desire of being.

Think about it right. Mushrooms are an entity, they exude an experience.
dmt can bring you into contact with many different kinds of entities.
dpt, while looking down the fractal you being the observer to the entity contact rather than the actual person going through the experience.

Now 4-ho-dpt, has very few reports. But it seems to have an entity, theres only a few reports on erowid about it.
But it seems seperated from physical being, spiritual essence, in order to actually have the magnitude, i.e. letting the genie out of the bottle.


So, now im sure many of you if you ever had any interest in studying mycology which i am starting with liquid culture.
now i havnt done any experiments with this yet. But i've certainly done alot of experiments with mycology.

Now, my understanding is the mycelium consumes its food whole if it runs into a tryptamine this can in theory convert said tryptamine into the 4-ho\\4-po counterpart. I want to fuse these two essences in a magnitude which is considered strong. i dont want to have to have to much back 14 grams to get a 70mg experience.

So is not liquid culture a form of substrate. especially when you are adding nutrient media like soy peptone and the like.
If i was to put the DPT hcl into the liquid culture along side nutrient media and then expanded mycelium from agar plating into said culture.
would it incorporate the dpt into its genetic make up? Thus i would have a jar of mycelium with the ready backbone to produce said fungi?

Anyways... heres some pictures. I've succeeded in doing full room spreads of fungi rather than fucking tubs.


Anyways with mushrooms being legal in denver and ALL Tryptamines pretty much still being legal in canada.
its time to get weird kids.

 

[F.U.B.A.R.]

Wow, that's an impressive setup you've got there mate!

However, I don't understand exactly what you're trying to achieve. Are all of those hallucinogenic species in their own right to which you're trying to alter the type of hallucinogen they contain, or are you trying to create hallucinogenic versions of non hallucinogenic species?

 

[Psychonautical]

okay, so out there on the internet, there exists an article by alexander shulgin in which he details how to use mycelium from psychedelic fungi as a biochemical forge for tryptamines. meaning you put the tryptamine into the substrate and when the mycelia comes into contact with it because it is using less energy to produce the 4-po-dmt is then produces more.

Now the article gets interesting when he starts talking about adding exotic tryptamines to the substrate,
like
AMT
DIPT
5-meo-dipt
DPT ect

there has been alot of speculative analysis. There is a really good german article about using Diethyltryptamine. how said mushrooms yielded 4-po-det.

Now 4-ho\\-4-po dipropyltryptamine sounds like a biochemical entity from alot of the trip reports i've read.
I literally believe it is lacking physical essence and spirit in order to fully be appreciated in spectrum.



There has been alot of research and some speculation as to if you were to lets say for example,
put tryptophan or tryptamine into the substrate. Would that increase the amount of psilocybin.
Its been done and yes it does work.


now heres the clinch right.

ive got a few grams of dpt kicking around. This concept or idea has been on the internet and debated on the shroomery and the hive for years now. I havnt done it, i havnt spoke to anyone to has succeeded. But im asking out to this old forum i used to go on if there are any mycophiles\\human lab rats who've experimented with this and had great success.

i use liquid media as my starting culture. i.e. water, honey, malt nutrient, soy peptone, and an antibiotic too prevent bacterial contamination.

Now, everyone suggests putting the tryptamine into your bulk substrate, sterlized media, i.e rye grain or coco coir or whatever the fuck.

If i made a honey, soy peptone, dipropyltryptamine sterile solution and then used living mycelium from an agarplate.
In a sterile setting and added said living mycelium to said solution. Do you think it would incorperate it.

I mean i might be the only asshole out here trying this shit out for kicks. who the fuck knows.

 

[Xorkoth]

Hey what's up man, long time no see, I'm always glad to see old BLers check in because so many have passed on.

He's talking about the research done that shows that adding any unsubstituted tryptamine (DiPT is what the research was done on, but it could be MET, MiPT, anything perhaps) to the substrate of psilocybin mushrooms will cause the mushrooms to convert that material into the 4-HO substituted version, and presumably also the 4-PO ester (which is what psilocybin is, 4-PO-DMT, vs psilocin, 4-HO-DMT). It's been shown to work with DiPT and there's no reason to think it wouldn't work with others. Since personally I can confirm that not only is 4-AcO-DMT distinct from mushrooms, but pure 4-HO-DMT is distinct from mushrooms (and 4-AcO-DMT). Additionally, different species of mushrooms (cyanescens, cubensis, etc) have a different makeup of effects, though they're all substantially similar.

Personally I'm really curious about your results. Correct me if I'm wrong but you're trying to investigate the differences in species in the makeup and nature of the results of adding different base tryptamines?

Actually maybe that's an additional variable.

Someone on here relatively recently added a different tryptamine into substrate and subjectively confirmed it worked/was pretty identical to the pure synthetic version that was expected to result. Hardly scientific but it's something. There is, after all, actual research to show that DiPT will be consumed and altered in this way.

 

[D Kaill]

Hi Psychonautical. We're in the same city and joined around the same time. I was "Aldousage" then, but can't remember my password and the email address has been defunct for many years now, so I had to sign up fresh today. Xorkoth might remember me as we corresponded off-site several times.

 

[Psychonautical]

hahahahah Xorgoth, you sir are correct. But lets not get into species yet.

i mean there has literally been so many reports over the years about this exact topic. But never one, that ive read about anyone whose had any kind of great success. Like you'd think fusing synthetic and organic would be something that would like nerds like us would just freak out.
But i mean, what happened, did the people doing this research 11 years ago die off?
Not succeed?
crack the enigma code and return back to a time of nephilem and 6 foot tall phosphorescent mushrooms?

Im in a position to start experimentation now. But, any old heads who did some of the research like what i am starting i'd like some qualatative and quantatative analysis i guess you would say,


I mean i was reading about being using ecoli to break down LSA to create a more open position on the amine group in order to find a way to actually incorperate that tryptamine into the mycelium.

But then boom, what the fuck happened? Research Chem companys got all stimmy, everyone got all tweakym then fentanyl analogues emerged on the internet, and now here we are. Anyways...

I pulled my head off the internet and did some real reality shit for awhile,
i.e. growing mushrooms. Now, I'm sitting here with mittens of tryptamines wondering what kind of bio-organic infused mutantly spiritual monsters i can create to undo the damage the egregoire of the internet has created in our society and culture. hahahah


Following me still?

So yeah, liquid culture, nutrient media. I dont see why the fuck it would have to be grain.
Why can't it be suspended in solution and then you just add living mycelium.

You aren't going to know if it works until you fruit it. But to me that seems smarter than dissolving a bunch of dpt into rye grain or bulk substrate

 

[Xorkoth]

I can't see why it wouldn't work as well or better in a nutrient media. You're right, the only way to know is to try. It would be good if you could actually get your results lab tested to really see the reality of what's happening. You could really contribute to scientific understanding if you do that. My hat is off to you, sir.

Hi Psychonautical. We're in the same city and joined around the same time. I was "Aldousage" then, but can't remember my password and the email address has been defunct for many years now, so I had to sign up fresh today. Xorkoth might remember me as we corresponded off-site several times.

Haha hey what's up man? I certainly remember you. If you want, you should post in the social thread or you could PM me too, curious how things have been going.

 

[Psychonautical]

would you count me doing an extraction on the fungi myself.
i've got few different distillation apparatai i can use in cold chambers and vaccumes.
ive got a friend who has a raman, hand held mass spec.
unless you know a lab, because i mean if this experiment works, im going to consider it my version of splitting the atom.

i'll even do a pictorial.

the other good news is
dipropyltryptamine hcl has a melt point of 352 farenheit,
and sterilization occurs at 100 farenheit below that. So there is literally no chance it would get broken down.

 

[cj187]

Making 4-HO-DPT from DPT will work. Jochen Gartz, the guy who did the experiment with DET, also did it with DPT and DiPT and has published papers about it though they're hard to find. I think he filed a patent for his process in Germany. I can tell you from personal experience that growing mushrooms with DiPT yields 4-HO-DiPT mushrooms.

I don't understand why you want to grow the mushrooms from liquid culture instead of grain. I know it's possible to grow mushrooms from liquid culture, but isn't the yield from that really terrible compared to traditional substrates? I've seen it talked about talked about on the Shroomery forums, and people concluded that liquid culture is basically worthless as a growth medium.

 

[Xorkoth]

From what my friend who got really into growing mushrooms said to me, he prefers liquid culture. But I think all he was doing was innoculating a sterilized sugar and water medium with spores and basically making a suspended mycelium culture that has a sealed lid that can be pierced with a needle without breaking the seal, and using a magnetic stirrer to stir it, and using that as an infinite source of spore syringes, innoculating cakes and then growing in casing in tubs.

I mean if you have access to sufficient equipment to determine what's in there then yeah it counts. I was thinking if you could send to a lab, it might cost some money but you could get precise results like content/ratios of specific esters and such.

 

[Psychonautical]

liquid media is used to expand culture.
my idea here is to have the tryptamine already in the nutrient liquid.
put the mycelium in there, let it gobble that up,
then put it to agar, grow a mushroom, clone it.
use then said clone to make another liquid culture,
then do a grain spawn test batch.

I dont wanna grow a bunch of duds.

 

[Xorkoth]

If you look up the method used in the studies done on this by Jochen Gartz, it would certainly work.

 

[cj187]

Oh okay, I thought you meant you wanted to use liquid culture as the main substrate instead of using it to colonize another substrate. Still, it sounds like what you're trying to do won't work. You need to have DPT in the substrate. Exposing mycelium to DPT doesn't alter it's properties.

 

[Psychonautical]

but what is causing the mycelium to grow.
the growth medium in it swirling around in.
i am using soy peptone which is basically the digestive amino acids of a bean at 5grams per L
so wouldnt the dpt also be consumed due to its existence in said media.

Like by your logic if i whipped up a batch of agar with dpt in it, which is a substrate, which you can grow fungi from, it should in theory technically work.

 

[Xorkoth]

I think, I'm not sure though, I've only grown mushrooms twice and never got into more advanced techniques, not even agar.

 

[cj187]

It will produce 4-HO-DPT as long as it has a supply of DPT available to it. If you have a liquid culture or agar with DPT in it then that mycelium would be able to make 4-HO-DPT, but if you use that to colonize another substrate it will run out of DPT and start producing psilocybin again.

 

[OntarioGuy]

Are u going to have any of the mushrooms tested for tryptamine content? I thinks that's the only way we could be truly sure. I don't think ingesting them and noting any difference would be case closed. It could help as an indicator if you are very good at distinguishing between 4 subbed tryptamines I guess.

But it would be very interesting if you were to have them analysed in a lab for the drug content

 

[DrumTripper]

That is exciting! I’m glad someone is trying these things. You’d be a hero to eventually create something like an LSA/H containing mushroom.
I love the sound of the bio-forge, churning out tryptamine varieties!
This is serious, but it is so amazing, sounding almost like some ancient magic or something. Like these mushroom masters in Elderscrolls growing houses from mushroom fibre.
Keep us posted, please!

 

[Xorkoth]

It woudn't just contain LSA/LSH, if it was possible for that to work it would add a 4-HO to it, perhaps. It would be something brand new, never seen before.

 

[Psychonautical]

It will produce 4-HO-DPT as long as it has a supply of DPT available to it. If you have a liquid culture or agar with DPT in it then that mycelium would be able to make 4-HO-DPT, but if you use that to colonize another substrate it will run out of DPT and start producing psilocybin again.

But if you have a fungi that is going to produce said 4-ho-dpt,
how would it reconvert?
If you cloned a mushroom that contained the bi product.

Wouldnt that mushroom then be a 'new species'