Xorkoth
Bluelight Crew
Yep, that'll work. 

I had a sub-par DMT trip and I'm wondering if it was the DMT itself (harsh yellow batch) or tolerance from a long trip the day before? Does anyone have experience taking DMT the day after tripping and did you notice decreased effects?
Madrus - it is likely due to the DMT itself. Yellow batches are usually fairly impure. Was it yellow crystals or yellow powder or solid or what? I have no problem tripping on DMT the day after tripping on anything else. I have never NOT been able to get high as long as I have great material to begin with.
endlessness said:4 FASI white
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Extraction
A/B on ad*e*nium bark, vinegar and lye, pulled with limonene at room temperature, salted with FASI. Only retrieved the FASI that were on the sides of the container, white crystals, not the yellow accumulation on the bottom. Only from first pull.
TLC
Results
Seemed to have DMT, NMT (and fumaric acid)
PDMAB-TS turned DMT and NMT yellow, did not have apparent reaction fumaric acid
GC-MS
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Results :
DMT, 2MTHBC, and limonene traces in the respective ratio of 319: 2.6: 0.1
Plus one unidentified insignificant trace component with too weak signal to tell what it is.
Raw Agilent *.MS file, plus pictures of mass spectra of the beta carb and limo is attached for those who want to double check the work.
Discussion and Questions:
* FASI precipitation yields only one beta-carboline trace, which does not seem to shine on UV ? This is interesting that there is another betacarboline or two that have not been described before in mimosa analysis. Its also interesting that they do not precipitate with FASI but do migrate to FASW and are present with xylene evap/jungle.
* Again, NMT is co-chromatographing with DMT and hence doesnt appear as distinct peak, check post in page 2
* Can these b-carbolines be active? Notice they are only in very trace amounts, here 2MTHBC is in less than 1%.
Possible ways to answer questions:
NMR, maybe some liquid chromatography. Will see if my friend can NMR it... liquid chrom, only next year.. Concentrating these b-carbolines with column and bioassaying would be interesting.
endlessness said:6- FASI - Yellow container-bottom accumulation
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Extraction
A/B on ad*e*nium bark, vinegar and lye, pulled with limonene at room temperature, salted with FASI. Only the yellow part that accumulated on the bottom of the container on the first pull (Same as 4- FASI white) was separated and analysed. This bottom yellow accumulation is due to small water content of IPA or that was left over from washing limonene before. As soon as one adds the FASI, if one sees drops/goo on the bottom, decant and let FASI fumarates crystallize slowly, this will separate.
TLC
Results
Seemed to have small amount of some beta-carboline, DMT, NMT (and fumaric acid)
PDMAB-TS turned DMT and NMT yellow, did not have apparent reaction with b-carboline, neither with fumaric acid
GC-MS
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Results :
DMT, 2MTHBC, MTHBC, another possible b carboline (edit: dozuki in post 66 and 67 of this thread suggests its a yuremamine degradation product) and limonene in the respective ratio of
103: 10: 2.4: 1: 6.8
Plus unidentified insignificant trace components with too weak signal to tell what they are.
Raw Agilent *.MS file is attached for those who want to double check the work.
Discussion and Questions:
* FASI "goo" that goes to bottom of container when using wet IPA has very similar alkaloid profile to FASW and jungle results are very similar alkaloid-wise than jungle spice. It is interesting to note that, as predicted, this water on the bottom of the container has pulled the other beta-carbolines that do not precipitate with propper dry FASI. A potential idea to further concentrate these other beta carbolines would be doing FASI and after retrieving crystals, washing with water to retrieve other b-carbolines.
* The goo had clearly much more limonene quantity than the clean FASI. While this wet yellow FASI one has nearly 7% of dmt weight in limonene (it was clearly wet as I took it to analyse), while the other clean FASI had 0.03% of limo weight. A little cold/dry acetone or IPA washing on wet orange-smelling fumarates would go a long way if you want to remove limonene leftovers.
* Again, where is that NMT that appears on the TLC (it should appear just after DMT, at around 5.7min)?
endlessness said:So i've found out why the NMT is not appearing. It basically co-chromatographs with DMT in this column/system we're using. It does appear as separate peaks when NMT is in large quantities (for example in Acacia confusa), but in very small quantity it appears under the curve area of DMT and the AMDIS program doesn't detect it as a separate peak (but if you go manually just before DMT, you can see in the ion scans that there are the two main peaks at 131, 44 and 77 (and the molecular peak at 174). So there you go, indeed those spots under DMT in TLC were NMT
A fantastic drug for me every time. reinvigorating, shocking, awe-inspiring, a real teacher. to be treated with real respect.
Potentiating DMT...that gave me a good laughMost intense DMT trip yet: 25mg + Nitrous right after exhaling.
WOW. I can't even describe it. Definitely potentiated it, that much is certain.