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  • Trip Reports Moderator: Cheshire_Kat

Bufotenine-first time-bioassay of bufotenine

Limpet Chicken

Bluelighter
Joined
Sep 12, 2004
Messages
1,266
Location
Right behind you.....
I just did a quick bioassay of bufotenine, extracted from Anadenanthere Colubrina (there is a remote possibility it may have been A.Peregrina, there is some controversy as to the identity of Anadenanthera species seeds available now)

Approximately 10mg of the freebase was insufflated in 2mg bumps, spaced 20 seconds apart, to gauge effectiveness, very little burn was notices, for maybe 5 seconds.

A peculiar sense of calm was noticed, paradoxically, I also felt somewhat stimulated and very clearheaded, some degree of tingling and prickling sensation, along with pounding heartbeat was noted also.

Effects lasted roughly 25 minutes.

Anadenanthera Colubrina seeds contain almost entirely bufotenine, with small amounts of 5-MeO-DMT, I believe the amount of 5-MeO-DMT in the small sample I insufflated to be almost insignificant.
 
Any plans to try this by either vaporization, or orally? The question of bufotenine psychoactivity sure is facinating. While classic 'experiments' with bufotenine in helpless mental patients failed to show any significant psychedelic effects, J. Ott claims that bufotenine isolate from A. Peregrina (verified by GCMS) is quite psychedelic when smoked, insufflated, or taken orally. However, some feel Ott is incorrect in reading the analysis...and possibly isobufotenine (6-hydoxy-N,N-dimethyl-1-indolethylamine) is the real active principle.

I sure don't know either way, but I do have a small sample of crystalline 'bufotenine' freebase which was extracted from A. Peregrina. While I have not yet personally sampled it, a good friend has and said something to the effect of...it felt like 'a' DMT, but it definitely was not DMT or 5-MeO-DMT. Probably won't be anytime soon, but I sure am going to give it a personal bioassay to make my own conclusions. :D
 
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I have heard from some quite knowledgeable people, that A.Peregrina simply isnt available, and ALL the samples of seed tested have proved to be A.Colubrina.

I have 4 plants (although one is earmarked to be a gift to my fiancee)
growing myself, of what I strongly suspect to be A.Colubrina C'ebil, possibly C'ebil ex Salta, they are maybe two months old, and the largest is already a foor tall, so I am going to have seeds to extrect by the bucketful =D

I believe these will be more promising to yield bufotenine for bioassay purposes, as in the Colubrina varieties, DMT is present in a trace, instead of 5-MeO-DMT, DMT of course, will probably be almost 100% inactive at the levels present in the extracted product.

I intend to go further with bioassay, because unlike your average mental patient (I am mental, yes, that may be, but I am not a patient incapable of giving an accurate account:\ ) although I really should get myself a set of scales, before going on with the experiments on a larger scale.

I have currently been extracting a given number of beans, and dividing the dosages into equal finely powdered piles, equivalent to the number of seeds from whence the alkaloid came, to avoid risk to health.

Check my gallery for some pics of the plants, and see wether they look like Peregrinas or Colubrinas to you, the biggest one there, is now double that size, in less than two fucking months=D
 
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