Thread: DNP Use Among Competitive Bodybuilders Common

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    DNP Use Among Competitive Bodybuilders Common 
    Performance Enhancing Drugs
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    CFC's Avatar
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    Mar 2013
    Koffin, Uganda
    We can draw that conclusion from the following paper published earlier this year:

    Wastewater-based tracing of doping use by the general population and amateur athletes ( 2018 )

    The present study investigates the applicability of the chemical analysis of wastewater to assess the use of doping substances by the general population and amateur athletes. To this end, an analytical methodology that can identify and quantify a list of 15 substances from the groups of anabolic steroids, weight loss products, and masking agents in wastewater has been developed. The method uses solid phase extraction to increase the detection sensitivity of the target analytes, expected to be present at very low concentrations (ng L−1 range), and decrease possible matrix interferences. Instrumental analysis is performed by liquid chromatography coupled to high-resolution mass spectrometry, allowing data acquisition in both full scan and tandem MS mode. The method has been successfully validated at two concentration levels (50 and 200 ng L−1) with limits of quantification ranging between 0.7 and 60 ng L−1, intra- and inter-day precision expressed as relative standard deviation below 15%, procedural recoveries between 60 and 160% and matrix effects ranging from 45 to 121%. The stability of the analytes in wastewater was evaluated at different storage temperatures illustrating the importance of freezing the samples immediately after collection. The application of the method to 24-h composite wastewater samples collected at the entrance of three wastewater treatment plants and one pumping station while different sport events were taking place revealed the presence in wastewater, and hence the use, of the weight loss substances ephedrine, norephedrine, methylhexanamine (DMAA), and 2,4-dinitrophenol. The use of these stimulants was visible just prior and during the event days and in greater amounts than anabolic steroids or masking agents.

    This is a graph from the paper. See if you can guess which day the contest was held on

    I mean, it's hardly surprising given how rife DNP use already is, but interesting given how much competitors and other bodybuilders on forums pretend they don't use it.

    However, it could also explain some of the subjective increase in shitty contest condition we've seen in recent years, since DNP typically leaves an ugly layer of subcutaneous water that (at least from my own experience and with my clients) you can't easily get out with diuretics.

    Last edited by CFC; 12-10-2018 at 19:05.
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