Greetings, this is my first post on this forum and researching different teks and ways to grow mushrooms in monotubs, I have started my first "batch". I have a pretty laid out/written out procedure for every step of the process, but I am still stuck on the slow or no growth of the mycelium within my substrate jars. Below is my explanation regarding situation regarding my inoculation process and colonization period. NOTE: THIS IS MY FIRST GROW ATTEMPT, bare with me
I used 6 half pint pre-sterilized subtrate mason jars that are filled with bee pollen, verm and some coco coir. There is a verm layer on the top of each, while the bee pollen is on the bottom portion of each jar. Each jar had 4 small, syringe sized holes made through the lid. I also used a Burma spore syringe (10cc).
When it was time to mix the spores into the jars, I sterilized the entire area, wore gloves, a hat, and had a sterile sterilite tub over my head to avoid any air contams that might reach the jars during the process. I also had a soaked (rubbing alcohol) paper towel for the syringe to rest on inbetween jars.
This is where I get worried.
Once I prepared all ingredients (sterilized and all), I began injecting spores. I shook up the syringe moderately beforehand, but still saw many of the spores were localized in the middle. Nonetheless, I was confident there would be enough spores spread out in the syringe that it would still work. I ended up injecting roughly 1.5cc-2cc of spore juice into each jar (WHICH IN RETROSPECT IS A LOT), but I felt it might speed up colonization and I needed to use the syringe anyways. Around my 3rd jar, I saw that a majority of the spores that were once localized were FINALLY being pushed out. I am very nervous that I put too many spores in one jar, and left far too few for any of the others. After I finished inoculating, I covered them all with tin foil and a rubberband for the night, in a warm/dark place.
The next day I picked up micropore tape and replaced the tin foil and rubberband on the tops of each with tape over the 4 holes for FAE. I have them now placed in my closet, with a space heater nearby that maintains a temp from 80-90 degrees F. The space heater fluctuates quite a bit, and I am worried there also isnt enough consistent heat. Humidity in the closet is also very low (10-40%). It has been 5 days now and not a single bit of mycelium is forming. The jars themselves are sitting in a contractor bag with tin foil lining the edges to trap heat. The bag itself is opened enough for FAE, but closed enough for no light to enter. I have been checking on the jars once a day since I inoculated.
NOTE: during the moving process/replacement of tin foil with micropore tape, the jars themselves were shaken and slightly tipped unintentionally.
Overall, I feel that only one jar will actually colonize, and that I fucked up the other 5 and wasted far too many spores. However, I am trying to remain positive and confident that the spores will do its job over time and germinate no matter how many are there. I just need some advice on whether I should retry with another batch of jars and different syringe (also shaking the syringe far more and being more precise). Should I wait it out a few more days? Is humidity a big factor when colonizing? And, would any of the above information/procedures worry you if this were to happen to you. I am open to any and all comments, my apologizes if there is already a thread for this, but I have been searching forever and haven't found anything this specific.
Greetings intrinsicnature12 - Welcome to Bluelight.
Jekyl Anhydride has provided some useful links in the posts above, and the search tool in the Psychedlics Drugs sub-forum may prove useful to you. I wish i could help more, but i am hopeful someone else here can.
If you still can't find your answer, head over to the Psychedelic Drugs sub-forum and start a thread there.